Product
DIAKEY REALcheck COVID-19(nCOV) Detection Kit
DIAKEY® REALcheck COVID-19 (nCOV) Detection Kit
DIAKEY REALcheck COVID-19 (nCOV) Detection Kit is in vitro diagnostic reagent to qualitative detection of RdRp, N gene of COVID-19 by Multiplex OneStep qRT-PCR.
Detection Target gene
- RdRp (ORF1ab) gene
- ORF8 (COVID-19) gene
- RP gen
Contents
| 2X OneStep qRT-PCR Master Mix[For Probe] | 1 mL * 1 ea |
| PM(RdRp/ORF8/RP Primer & Probe) | 0.2 mL * 1 ea |
| PC(RdRp/ORF8/RP Positive Control) | 0.8 mL * 1 ea |
| RNase free water | 1 mL * 1 ea |
The Kit includes 2X OneStep qRT-PCR Master Mix(Including RTase, DNA Polymerase and RNase inhibitor) and PM(Primer Mixture). We also provide a PC(Positive Control(2019_nCoV)), which allows us to compare the results with clinical sample results to ensure correct interpretation of the results.
Procedure
- 1. Sample Processing
Sampling swabs/sputum are pooled in a sufficient volume of sterile Buffer - 2. RNA reparation
DIAKEY REALcheck Viral DNA/RNA Prep Kit can be used - 3. Reaction Set-up
qRT-PCR Reverse Transcription and Amplification - 4. Data Analysis
validity and qualitative result
| Type | FAM | Texas Red | HEX(VIC) | Result Analisys |
|---|---|---|---|---|
| RdRp gene | N gene | RP gene | ||
| PC(Positive Control) | + | + | + | Valid |
| NC(Negative Control) | - | - | - | Valid |
| Sample1 | + | + | +/- | Positive |
| Sample2 | + | - | +/- | Positive |
| Sample3 | - | + | +/- | Positive |
| Sample4 | - | - | +/- | Negative |
CAUTION!!
It is recommended to proceeding without interruption to avoid any degradation of isolated samples and reagents.
If necessary, store isolated RNA at -80℃ to -70℃ before use and avoid repeated freezing and thawing.
Sensitivity
Order Info
| Product | Cat No. | Box Size(mm) | Shelf Life | Storage |
|---|---|---|---|---|
| DIAKEY® REALcheck COVID-19(nCOV) Detection Kit | MF01 | 30g, 70*50*55 | 6 months | -20℃ to -15℃ |